Natural cure for Diabetes

Is Moringa Oleifera a natural cure for Diabetes?

Natural cure for Diabetes

Diabetes is a disease which is characterized by problems involving the hormone insulin. In healthy people, the pancreas releases insulin; insulin then works to help the body use and store the fat and sugar that is derived from the food that people eat. With diabetes, insulin can be compromised in a couple of different ways. In some cases, the pancreas doesn’t produce any insulin at all. Other times, the body does not react in the right way to insulin – this is known as “insulin resistance.” Finally, diabetes is sometimes characterized by a pancreas that produces small amount of insulin. 

There are three main types of diabetes: 

• Type 1 diabetes 

• Type 2 diabetes 

• Gestational diabetes 

Moringa Oleifera is a nutrient plant that can help to maintain normal blood sugar levels. It holds so much promise for those who suffer from diabetes. This is primarily because of its many amazing, natural benefits. Moringa Oleifera has been shown to naturally boost the immune system, which usually becomes compromised in those who suffer from type 1 and type 2 diabetes. Moringa Oleifera has also been shown to possess many key anti-inflammatory benefits; diabetes often causes circulatory problems which can be managed through anti-inflammatory supplements. There are no negative side effects associated with Moringa Oleifera use, meaning that it is a safe, natural way for people to manage their blood sugar and care for their diabetes symptoms. It’s just one more option for the many people who have to cope with this serious condition 

Moringa Oleifera as a rich source of ascorbic acid helps in insulin secretion. It is interesting to note that certain nutrients like vitamins B1, B2, B12, pantothenic acid, vitamin C, protein and potassium – along with small frequent meals containing some carbohydrate – can actually stimulate production of insulin within the body.

Effect of Moringa oleifera Lam. leaves on hyperglycemic

Effect of Moringa oleifera Lam. leaves aqueous extract therapy on hyperglycemic rats

Moringa Oleifera: A treatment for Diabetes

MORINGA- THE MIRACLE PLANT!!! THIS IS A SURVIVAL PLANT AND WILL BE VITAL IN NEAR FUTURE!!!

Diabetes Treatment
Moringa Oleifera: A treatment for Diabetes

Diabetes is a disease that is characterized by problems involving the hormone insulin. In healthy people, the pancreas releases insulin; insulin then works to help the body use and store the fat and sugar that is derived from the food that people eat. With diabetes, insulin can be compromised in a couple of different ways. In some cases, the pancreas doesn't produce any insulin at all. Other times, the body does not react in the right way to insulin - this is known as "insulin resistance." Finally, diabetes is sometimes characterized by a pancreas that produces an insufficient volume of insulin. The Two Types of Diabetes It's important to understand that diabetes is a disease that has no cure. Once a person develops diabetes, they will suffer from the condition for the rest of their life. Although diabetes may be triggered by a variety of different phenomena involving the pancreas and insulin production - or lack thereof - it can also be divided into two distinct types. Type 1 Diabetes - Type 1 diabetes typically first arises in people under the age of 20, although it can happen at any age. Insulin-producing cells - known as beta cells - in the pancreas are completely destroyed by the body's immune system. In turn, the pancreas can no longer produce any insulin and insulin injections must be administered. Type 2 Diabetes - With type 2 diabetes, a person's pancreas still produces insulin; the problem is that it either doesn't create enough insulin, or the person's body is resistant to the insulin that is produced. Type 2 diabetes commonly occurs in obese and overweight individuals - usually over the age of 40 - and is sometimes called "adult onset diabetes." Managing Diabetes There is no cure for diabetes. However, there are several ways to manage the condition in order to keep insulin at the proper level. There are several different techniques and strategies for managing diabetes. Some of them include:  carefully monitoring one's diet in order to keep blood sugar levels in check;  using insulin injections as needed to maintain optimal levels in those whose bodies don't produce the hormone;  keeping a close eye on blood sugar levels by using special kits that measure insulin and sugar in the blood; and  following an exercise routine in order to keep blood pressure levels in check. Moringa Oleifera: A Natural Treatment for Diabetes As with any disease or condition, doctors and researchers are constantly seeking new ways to treat and manage diabetes. People are more concerned about using harsh, synthetic medications than ever before, which is what makes the promise of a tree called Moringa Oleifera all the more exciting. Moringa Oleifera is a tree that is originally native to India, but is now grown across the globe. As it happens, people in many developing countries - particular in Africa - have been using Moringa Oleifera to treat and manage the symptoms of diabetes for years. Why does Moringa Oleifera hold so much promise for those who suffer from diabetes? Primarily because of its many amazing, natural benefits. Moringa Oleifera has been shown to naturally boost the immune system, which usually becomes compromised in those who suffer from type 1 and type 2 diabetes. Moringa Oleifera has also been shown to possess many key anti-inflammatory benefits; diabetes often causes circulatory problems which can be managed through anti-inflammatory supplements. There are no negative side effects associated with Moringa Oleifera use, meaning that it is a safe, natural way for people to manage their blood sugarand care for their diabetes symptoms. It's just one more option for the many people who have to cope with this serious condition. [link to www.teampure.org]

http://www.godlikeproductions.com/forum1/message1950796/pg10

Moringa Oleifera as Treatment for Gout & Arthritis
For many centuries Moringa Oleifera was used in traditional medicine, such as the "Ayurveda", as a treatment Arthritis and Gout. It was believed that Moringa Leaf Powder reduce inflammations and pain caused by these conditions.
In 2008 the Faculty of Medicine Health Sciences of the Putra University in Malaysia, published a research aimed to test if Moringa poses any Antinociceptive and Anti-Inflammatory activities, the same activities achieved by the drugs used in modern medicine such as NSAID (non-steroidal anti-inflammatory drugs). The research found that the Moringa leaves posses substances that have antinociceptive and anti-inflammatory activitie; in fact they found that it has high quantities of these substances. This means that Moringa Oleifera use in Indian traditional medicine as a treatment Arthritis and Gout was just.
The effects of the Moringa is suppressing the COX-2 enzyme. This enzyme is responsible for inflammation processes and pain. Usually the drugs used to suppress this enzyme are NSAID such as Voltaren, Nksin, Adoil, ARCOXIA, Kaspo etc. The side effects of these products include
risk for stroke, kidney problems, blood vessels problems, stomach problems and the list goes on. Combine the risks from the side effects with the existing condition causing the need to take the NSAID and the presence of pain combined with an inflammatory issue, and the risks for medical complications grow. Moringa Oleifera in pure natural tree that has been used as food and as medicine along history, this means that it is much safer to use than NSAID but with similar effectiveness and less toxicity. [link to www.teampure.org]
Moringa oleifera (Moringaceae), a plant indigenous to India is considered by botanists and biologists, a miracle of nature. A hope for combating world hunger. Rich in vitamins and minerals, it has:

a) seven times more vitamin C than oranges; 
b) Four times more calcium than milk; 
c) Four times more vitamin A than carrots; 
d) Three times more potassium than bananas, 
and) two times more protein than milk (27% protein equivalent to the beef), 
e) More than spinach iron, 
f) present Vitamins A, B (thiamine, riboflavin, niacin), C, E and beta carotene. 
g) minerals: Phosphorus, Iron, Selenium and Zinc.

Internet search will show hundreds of references. It is considered medicinal. Studies have shown its effectiveness in dozens of diseases: it is anti-diarrheal, anti-inflammatory, anti-microbial, anti-spasmodic, anti-diabetic, diuretic, vermífuga (flowers and seeds).

POSSIBLE USES OF PLANT

SEEDS - From its seed is extracted an oil similar in quality to olive oil. As the only known plant that blooms all year, is also considered honey, suitable for beekeeping. His honey is considered high value medicinal and reaches the European market. Intensive production of flowers and seeds, recent studies indicate its biodiesel plant to extract from its seeds.

The green seeds can be cooked, equals white beans, soybeans, etc.., And served as salad.

LEAVES - The leaves and flowers are edible for humans and animals. They may also be used for continuous use in tea. It has been used to feed sheep, goats, hens, rabbits and cows. The association recommends the Brazilian caprinocultores.

FLOWERS - From flowers to make a dish popular in Indonesia and East Timor, called makansufa , flowers are fried in coconut oil, and dipped in coconut milk, and eaten with rice or corn. The leaves and flowers can also be consumed in vitamins or juices with other vegetables such as beets, carrots, or fruits like orange, apple, melon, papaya, cashew, pineapple. etc.. Can also be used for continuous use in tea.

The flowers can also be used in a medicinal tea, recommended for colds, for popular use in several countries.The juice of the flowers or leaves, you can make soups or sauces, in its natural way to preserve vitamins and minerals. It is excellent treatment for weight reduction, and it is rich in nutrients, facilitates a food reeducation without injury to the body and metabolism.

Its flowers are used to feed bees like Europe (Apis) or native stingless. Produce much nectar to feed the bees, blooming year todo.De their leaves, flowers or seeds, you can extract a product used as a clarifier in the treatment of drinking water, similar to products used by companies to chemical treatment water. The leaves soaked in pools of muddy water cause rapid cleaning. If not contaminated, is fit for consumption. In northeastern Brazil this plant is already being used for this purpose.

PODS - The new pods can be cooked, equals green beans or asparagus. And thus widely used in Haiti.

PEEL - From their shells to make crafts, they are very soft and own to shape and make baskets, weaving, etc. .. Can be processed to extract a fiber to produce carpeting. Its sap tastes sweet. The bark and resin, is tannin, which is utiulizado for tanning leather for making shoes, bags, clothing, etc..

POTATOES -You can plant it in beds, as a vegetable, and when the plant reaches about 30 cm, pulls up his foot and pulls a potato for food consumption. It tastes like horseradish, near the radish, and potato sap, has all the vitamins concentration in plants. This potato can be eaten in salads or sauteed. Or even fruit juices or vegetables. After this period of 30 days the potatoes disappears and becomes the root of the plant.

ORNAMENTAL - In many countries, Moringa is planted as an ornamental plant because it produces flowers throughout the year, being the only known plant with this capacity.

PULP - Its wood is soft, but is excellent for producing pulp for paper manufacturing.

ADDITIONAL INFORMATION ON USE

HUMAN - Due to its nutritional properties, can be used in the treatment of malnutrition, it is rich in protein, vitamins and minerals. It can also be used to combat obesity and high cholesterol, replacing it with equivalent nutrition, but with more vitamins and minerals, meat and various other fattening foods or are rich in saturated fats.

FEED - Can also be planted as forage to feed sheep, goats, rabbits, hens, dairy cows. Plant the seeds every 80 cm. When the plant reaches 80 cm in height, cuts off the hands. After budding new, multiple shoots will emerge. When they reach 30 centimentos, cut it back all the pointers, so there is a new sprout. Once the plant becomes more rounded. After this second break pointers, can be cut shoots and remove the sheets to serve as food. The concentration of vitamins and minerals, is a noble food which helps reduce the cost of raising.

MEDICINAL USE - In Africa, millions of people with HIV and AIDS, has been a weapon in combating the effects of this debilitating disease, it is rich in protein, vitamins and minerals, as it is powerful weapon against malnutrition in chronic many parts of that continent.

Positive results occurred in the treatment of prostatitis, prostate cancer, rheumatism, tumors, lupus erythematosus, arthritis and other autoimmune diseases, hypertension, hepatitis, gastrointestinal motility, Epstein-Barr virus, epilepsy, chronic fatigue, illness caused by the treatment of cancer, prenatal care, glaucoma, malnutrition of adults and children, reducing obesity, cure gastro-intestinal irritation of skin diseases, bronchitis and inflammation of the mucosa in infants. The roots are laxative. The plant produces rejuvenating effect of the epithelial cells of the sexual organs and the brain.

Studies have shown its effectiveness in dozens of diseases: it is anti-diarrheal, anti-inflammatory, anti-microbial, anti-spasmodic, anti-diabetic, diuretic, vermífuga (flowers and seeds).

There is quote from the use of this plant for this purpose in the Bible, in Exodus 15:20-25. She is considered a miracle of nature, a true natural pharmacy.

There are hundreds of websites disseminating this plant and its products around the world. There are research institutes researching this plant and disseminating the results. Just to cite the research ^ ^ Moringa oleifera, thousands of results appear, in many languages. In our website at FACTS AND INFORMATION - RECOMMENDED SITES , there is a list of sites that can be visited, with a huge range of information about the plant.
http://moringatreeinfo.blogspot.in/2009/11/moringa-vitamins-in-treatment-of.html

Moringa Oleifera can help Diabetes

How Moringa Oleifera can help Diabetes

How Moringa Oleifera can help Diabetes

In healthy people, pancreas releases insulin which helps the body to use and store fat and sugar from the food we eat.

In diabetes, sometimes pancreas does not produce any insulin at all; other times, body does not react in the right way to insulin ( Insulin Resistant); finally, pancreas produces an insufficient amount of insulin.

Like arthritis, there’s no cure for diabetes.  Once a person develops diabetes, they will likely suffer the condition for the rest of their life.

But the GOOD NEWS is that there are several ways to manage the condition in order to keep insulin at the proper level:-

1)      Change the kind of carbohydrates you eat --- eat plenty of whole grain, veggies and legumes.

2)      Eat healthy monounsaturated fats such as olive oil and flax seeds.

3)      Lose weight sensibly

4)      Regular moderate physical exercises

5)      Consider supplementing your diet with Moringa Oleifera (Zija)….see below for more details…

How Moringa Oleifera (Zija) can help those suffering from diabetes?

First of all, Moringa Oleifera is used for treating diabetes by many developing countries, particularly in Africa, to manage the symptoms of diabetes for years.

Moringa Oleifera contains about 73% of monounsaturated oil that has the ability to regulate blood sugar levels and as a result, can reduce the harmful build up of sugar in the blood (over time, if uncontrolled, the harmful build up of sugar in the blood can lead to kidney failures, hypertension, blindness, dementia, and loss of circulation to legs and feet).  In addition, Moringa leaves also have a stabilizing effect on blood pressure and control of glucose levels.

Moringa Oleifera has been shown to naturally boost the immune system which usually becomes compromised in those who suffer type 1 or type 2 diabetes.

Moringa Oleifera has been specially formulated into a convenient drink called Zija “Smart Mix” by the Zija company.

Diabetes often causes circulatory problems which can be managed through anti-inflammatory supplement. And Moringa Oleifera (Zija) has more than 36 natural anti-inflammatory compounds.

http://allnaturalremedy.blogspot.in/2012/04/how-moringa-oleifera-zija-can-help.html

Moringa cures Diabetes

Moringa cures Diabetes

http://www.moringathai.com/index.php?mo=3&art=399600
moringo testimony for diabetes
http://www.youtube.com/watch?feature=player_embedded&v=f4N4F27HfXc

Diabetes Cured by Moringa

Diabetes is a metabolic disorder where the human body does not produce or properly uses insulin, a hormone that is required to convert sugar, starches, and other food into energy. Diabetes mellitus is characterized by constant high levels of blood glucose (sugar). Human body has to maintain the blood glucose level at a very narrow range, which is done with insulin and glucagons.

The abnormally high blood sugar (hyperglycemia) resulting from insufficient levels of the hormone insulin which is characteristic symptoms polyuria, polydipsia, glucoseuria, unexplained weight loss, and lethargy. There are two types of diabetes: Type I diabetes known as insulin dependent diabetes and Type II diabetes refer to insulin non dependent diabetes.  Diabetes II can be avoided through proper dieting, medication and regular exercise.

Diabetes will lead to complications such as retinopathy, neuropathy, nephropathy, cardiovascular problems etc., researchers recently reported that vitamin D is essential for the islet cells in the pancreas to be able to secrete insulin properly. The studies have shown that individuals with  low vitamin D levels experienced the worst blood sugar-handling problems and had a greater risk of developing diabetes. Moringa as a rich source of ascorbic acid helps in insulin secretion. It is interesting to note that certain nutrients like vitamins B1, B2, B12, pantothenic acid, vitamin C, protein and potassium – along with small frequent meals containing some carbohydrate – can actually stimulate production of insulin within the body.

Vitamin A as an antioxidant helps convert beta-carotene efficiently, which reduces the risk of blindness in diabetics. Vitamin B 12 can help people suffering form diabetic neuropathy. High dose supplements of vitamin C have been shown to prevent sorbitol accumulation and glycosylation of proteins, both of which are important factors in the development of diabetic complications such as cataracts. Studies have shown that a low vitamin-E concentration was associated with a 3.9 times greater risk of developing diabetes. Vitamin E reduces oxidative stress, thus improving membrane physical characteristics and related activities in glucose transport. This antioxidant promotes healing of diabetes-related lesions. Magnesium helps in the metabolism of glycogen, Mg works closely with vitamin B 6 to help the metabolic process with in the cell. Moringa has all these nutrients and helps the diabetic patient control their blood glucose level.

Evaluation of antidiabetic and antioxidant activity of Moringa oleifera in experimental diabetes

Evaluation of antidiabetic and antioxidant activity of Moringa oleifera in experimental diabetes

Evaluation of antidiabetic and antioxidant activity of Moringa oleifera in experimental diabetes
Oleifera, the widely cultivated species in India is an exceptionally nutritious vegetable with a variety of potential uses in treating rheumatism, venomous bites and microbial infections. The antidiabetic and antioxidant effect of methanol extracts of Moringa oleifera pods in streptozotocin (STZ)-induced diabetes in rats was studied. Methanolic extract of M. oleifera (MOMtE) was studied for antidiabetic effect on STZ-induced diabetes on albino rats. The protection was measured by changes in biochemical parameters of serum and pancreas tissue. Diabetic rats were treated daily with different doses of MOMtE, i.e. 15 and 3 mg/kg b. wt./day for 21 days. Two phytoconstituents, quercetin and kaempferol were isolated from the MOMtE extract and structures were determined using NMR and IR spectroscopy. The data of present study demonstrated that the progression of diabetes significantly reduced after MOMtE treatment. MOMtE induced a significant reduction in serum glucose and nitric oxide with concomitant increase in serum insulin and protein levels. Furthermore, MOMtE treatment increased the pancreas tissue antioxidant level with concomitant decrease in the level of thiobarbituric acid-reactive oxygen species. The histologic examination of pancreas of diabetic rats showed degenerative changes in the number of β-cells, while MOMtE treatment significantly regenerated the islets cells. Moringa oleifera exerts protective effect against STZ-induced diabetes. From present study it can be concluded that methanolic extract of M. oleifera possess significant antidiabetic and antioxidant activity and may be considered in isolation of active constituents for clinical studies.





http://scienceindex.com/stories/1972728/Evaluation_of_antidiabetic_and_antioxidant_activity_of_Moringa_oleifera_in_experimental_diabetes.html

Potential of Moringa oleifera Leaves in Chronic Hyperglycemia

Therapeutic Potential of Moringa oleifera Leaves in Chronic Hyperglycemia and Dyslipidemia: A Review

Therapeutic Potential of Moringa oleifera Leaves in Chronic Hyperglycemia and Dyslipidemia: A Review

Moringa oleifera (M. oleifera) is an angiosperm plant, native of the Indian subcontinent, where its various parts have been utilized throughout history as food and medicine. It is now cultivated in all tropical and sub-tropical regions of the world. The nutritional, prophylactic, and therapeutic virtues of this plant are being extolled on the Internet. Dietary consumption of its part is therein promoted as a strategy of personal health preservation and self-medication in various diseases. The enthusiasm for the health benefits of M. oleifera is in dire contrast with the scarcity of strong experimental and clinical evidence supporting them. Fortunately, the chasm is slowly being filled. In this article, I review current scientific data on the corrective potential of M. oleifera leaves in chronic hyperglycemia and dyslipidemia, as symptoms of diabetes and cardiovascular disease (CVD) risk. Reported studies in experimental animals and humans, although limited in number and variable in design, seem concordant in their support for this potential. However, before M. oleifera leaf formulations can be recommended as medication in the prevention or treatment of diabetes and CVD, it is necessary that the scientific basis of their efficacy, the therapeutic modalities of their administration and their possible side effects be more rigorously determined.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290775/

++

INTRODUCTION

Moringa oleifera, Lam (M. oleifera), also known as Moringa pterygosperma Gaertn, is a member of the Moringaceae family of perennial angiosperm plants, which includes 12 other species (Olson, 2002). Native of the sub-Himalayan northern parts of India, it is cultivated throughout tropical and sub-tropical areas of the world, where it is known by various vernacular names (Ramachandran et al., 1980), with drumstick tree, horseradish tree, and malunggay being the most commonly found in the literature.

Moringa oleifera is an edible plant. A wide variety of nutritional and medicinal virtues have been attributed to its roots, bark, leaves, flowers, fruits, and seeds (Ramachandran et al., 1980; Anwar et al.,2007; Kumar et al., 2010). Phytochemical analyses have shown that its leaves are particularly rich in potassium, calcium, phosphorous, iron, vitamins A and D, essential amino acids, as well as such known antioxidants such as β-carotene, vitamin C, and flavonoids (Bennett et al., 2003; Aslam et al., 2005; Manguro and Lemmen, 2007; Amaglo et al., 2010; Gowrishankar et al., 2010).

The therapeutic use of M. oleifera parts in the Indian subcontinent dates back to Antiquity. In other parts of the world, in sub-Saharan Africa in particular, such a use appears not to have been known by the wide public, as it is being promoted by diverse organizations as an untapped opportunity (Thurber and Fahey,2009; Torimiro et al., 2009). At the writing of this manuscript (April 2011), a search on the Internet, using the Google browser and the words moringa, drumstick, or malunggay as keys within page titles, reported nearly 90,000 websites. Most of these sites present this plant as a remedy to malnutrition and a vast range of ailments. On the Internet, M. oleifera is variably labeled as Miracle Tree, Tree of Life, Mother’s Best Friend, God’s Gift to Man, Savior of the Poor. In many regions of Africa, it is widely consumed for self-medication by patients affected by diabetes, hypertension, or HIV/AIDS (Dieye et al.,2008; Kasolo et al., 2010; Monera and Maponga, 2010). Yet, in a similar search of the PubMed database of biomedical publications, merely 163 papers were fetched; 76% of them were published in the last decade (Figure ​(Figure1).1). A similar search through Google Scholar produced about 1250 articles; the vast majority of these were in scientific journals not indexed in PubMed, and a good number in non-peer-reviewed book chapters, patents, citations, or other documents. By comparison, green tea, another plant claimed to be medicinal for a range of illnesses (Schneider and Segre, 2009), 1972 and 7240 publications were retrieved in a similar search in PubMed and Google Scholar, respectively. Clearly, in spite of the widely held “belief” in the health benefits of M. oleifera, the interest of the international biomedical community in the medicinal potential of this plant has been rather tepid.

Figure 1

Chronological trend of scientific publications on M. oleifera. The bibliographic search was conducted online in the PubMed database at www.pubmed.ncbi.nlm.nih.org/, using moringa, drumstick, or malunggay as title keywords. Article abstracts were reviewed ...

This report is a review of current data on the therapeutic potential of M. oleifera in chronic hyperglycemia and dyslipidemia. Chronic hyperglycemia is an indicator of diabetes mellitus (DM), and chronic dyslipidemia a risk factor for cardiovascular disease (CVD). These metabolic disorders are global epidemics (Yusuf et al., 2001; Wild et al., 2004). In developing countries, they are on the way to becoming as major causes of morbidity and mortality as infectious diseases, due to the progressive transition in these countries to a lifestyle characterized, among other aspects, by greater access to dietary calories and less demand for calorie expenditure (Hossain et al., 2007; Aje and Miller, 2009). Geographically, many of developing countries are located in the tropical and sub-tropical regions of the world where M. oleifera grows and is cultivated. If validated by medical science, dietary consumption of this plant could be advocated in these and other countries as an inexpensive prophylactic strategy against DM and CVD.

The review covers peer-reviewed studies of the therapeutic potential of M. oleifera in these pathologies. It focuses on studies involving experimental animal models and human subjects, in which leaves were used as the medicinal parts of the plant. Leaves were generally administered per os (p.o.) as powders or aqueous or organic extracts thereof, and, for experimental animals, at body weight-adjusted doses. A therapeutic effect was defined as a beneficial change in specific biochemical parameters of disease.

The review includes five sections. The first two sections are concerned with the scientific evidence of M. oleifera efficacy at correcting chronic hyperglycemia or dyslipidemia. They each begin with a brief description of main aspects of glucose or lipid homeostasis and its pathogenic disruption, so that the therapeutic efficacy of the plant could be subsequently evaluated based on current biomedical understanding. The third section attempts to correlate the observed therapeutic effects with the presence in the leaves of physiological properties or chemical constituents experimentally proven to offer such metabolic benefits. The fourth section briefly examines what is known of the possible toxicity of M. oleifera leaves. The final section identifies obvious gaps in current knowledge and proposes potential avenues for future investigation.

Go to:

METHODOLOGY

Two biomedical literature databases were searched: PubMed and Google Scholar (subsections: Biology, Life Science, Environmental Science, Medicine, Pharmacology, and Veterinary Science). The search was conducted using moringa or drumstick as a keyword within the title of the article. Articles dealing withMoringa varieties other than oleifera were excluded; as were articles in which the word drumstick referred to other subject matters (e.g., medical conditions or transcription factor) than the plant. All the remaining articles, including reviews, were critically read. Peer-reviewed articles presenting results of experimental studies, in animals or humans, on the therapeutic potential of M. oleifera leaf extract or powder in chronic hyperglycemia or hyperlipidemia were analyzed for inclusion in this review. The analysis focused on therapy subjects, galenic formulation, mode of administration, dosage, treatment duration, and measurable biochemical outcomes.

Go to:

THERAPEUTIC POTENTIAL OF M. OLEIFERA IN CHRONIC HYPERGLYCEMIA

Glucose homeostasis

Glucose is a major fuel for animal cells. It is supplied to the organism through dietary carbohydrates and, endogenously, through hepatic gluconeogenesis and glycogenolysis. Glucose absorption from the gastrointestinal tract (GIT) into blood is regulated by a variety of neuronal signals and enterohormones (incretins), as well as by meal composition and the intestinal flora. Glucose homeostasis reflects a balance between glucose supply and its utilization. Physiologically, this balance is determined by the level of circulating insulin and tissue responsiveness to it. Insulin is secreted by pancreatic islet β cells. It stimulates glucose uptake and utilization by tissues, especially by liver, skeletal muscle, and adipose tissue. It also suppresses gluconeogenesis in hepatocytes, while stimulating lipogenesis and inhibiting lipolysis in adipocytes (Gerich, 2000).

Hyperglycemia

An individual is diagnosed as diabetic when his blood glucose level is chronically ≥126mg/dL after an overnight fast, and ≥200mg/dL 2h after an oral glucose load of 75g (oral glucose tolerance test, OGTT; Alberti and Zimmet, 1998). Age, genetics, environment, and lifestyle influence the development of this pathology. The relative importance of these factors and their combinatorial effects are not yet fully understood. Two types of DM are commonly recognized: type 1 DM (T1DM) results from autoimmune destruction of pancreatic β cells and represents only 5% of all cases; type-2 DM (T2DM) is the most common form of the disease and the primary concern of this review.

In its early stages, T2DM is characterized by chronic hyperglycemia and hyperinsulinemia, due to loss of tissue sensitivity to insulin, and compensatory secretion of the hormone by islet β cells. Its progression involves a complex network of interacting cellular and physiological alterations leading to β cell failure. Glucotoxicity and lipotoxicity are the most commonly invoked mechanisms for this failure (Robertson et al., 2004).

Glucotoxicity arises from excessive uptake of glucose by islet β cells. The excess sugar drives glycation reactions and the mitochondrial electron transport chain, producing macromolecule-damaging reactive oxygen species (ROS), at levels beyond the antioxidation capacity of the cell. The ensuing oxidative stress impairs insulin synthesis and secretion, and initiates a cascade of cellular events that ultimately lead to apoptosis (Kaneto et al., 2007).

Lipotoxicity, on the other hand, results in part from the unresponsiveness of adipocytes to insulin, negating the ability of this hormone to stimulate uptake by these cells of non-esterified fatty acids (NEFA) that result from triglycerides (TG) lipolysis in circulation, and to inhibit lipolysis of endogenous TG to NEFA. Excess plasma NEFA impairs insulin secretion by β cells, stimulates gluconeogenesis by liver, and inhibits glucose disposal by skeletal muscle, further exacerbating hyperglycemia (Stumvoll et al., 2005). NEFA accumulation in the bloodstream is further aggravated by obesity, a condition characterized by an expanded adipose mass. Furthermore, adipose tissues, especially the visceral and deep subcutaneous ones, secrete pro-inflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor α (TNFα), which also contribute to tissue insensitivity to insulin.

Impaired TG storage into adipocytes facilitates the formation in the bloodstream of small, cholesterol ester-poor, TG-rich low-density lipoprotein (LDL) particles. Hyperglycemia promotes glycation of these particles, a modification that extends their half-life in circulation. These particles are prone to oxidation and are potent initiators of atherogenesis and its vascular damages (discussed in more detail below). Diabetes-associated neuropathy, retinopathy, and nephropathy are some of the consequences of these damages (Dokken, 2008).

Underlying these complex physiological changes are molecular alterations in the relative levels of expression and post-translational modifications of a wide variety of gene products, including surface receptors, second messengers and transcriptional factors.

Evidence of anti-hyperglycemic properties of M. oleifera

Moringa oleifera parts have been used in folk medicine for the treatment of diabetes (Dieye et al., 2008). Five studies aimed at verifying these properties using leaves were identified in the scientific literature: two were conducted in experimental animals (Ndong et al., 2007b; Jaiswal et al., 2009) and three in T2DM patients (William et al., 1993; Kumari, 2010; Ghiridhari et al., 2011). They are summarized in Table ​Table11.

Table 1

Moringa oleifera experimental therapy for chronic hyperglycemia.

Animal studies

In the study by Ndong et al. (2007b), Goto-Kakizaki (GK) Wistar rats were used as model of DM. GK rats spontaneously develop early glucose intolerance associated with impaired insulin secretion (Bisbis et al., 1993; Abdel-Halim et al., 1995). In an OGTT, overnight fasted Wistar controls or GK male rats were given 2g/kg of body weight (kg-bw) glucose by oral gavage, without or with 200mg/kg-bw of M. oleifera leaf powder. To determine blood glucose levels, vein blood was collected before gavage and at different times afterward up to 120min. Areas under the curves (AUC) were derived from the time courses of these levels. In the absence of treatment, fasting plasma glucose levels (FPG) and their post-prandial levels (PPPG) at 120min were greater (∼1.4× and 2.2×, respectively) in GK rats than in control rats. Treatment with M. oleifera leaf powder resulted in a lower glycemic response in GK and control rats. However, in GK rats, the treatment reduced AUC values by 23% (P<0.05); it did not significantly affect these values in control rats. These observations suggested that M. oleifera treatment improves plasma glucose disposal only in the diabetic rats.

In the study by Jaiswal et al. (2009), prediabetes and diabetes were induced in Wistar rats by intraperitoneal (i.p.) injection of 55mg/kg-bw of streptozotocin (STZ), a cytotoxic drug that selectively destroys islet β cells (Like and Rossini, 1976). Based on FPG (mg/dL), STZ-treated rats were classified as sub-diabetic (∼88mg/dL), mildly diabetic (∼190mg/dL), and severely diabetic (∼300mg/dL). A M. oleifera aqueous extract was administered to overnight fasted animals by oral gavage, at 100, 200, or 300mg/kg-bw. FPG was determined before treatment (baseline) and at various time points post-treatment. An OGTT was conducted 90min after the last time point. In normal rats, M. oleiferatreatment lowered FPG at all doses in time- and concentration-dependent manners. Six hours after administration, 200mg/kg-bw of M. oleifera extracts lowered FPG by about 26% (P<0.05), compared to baseline levels or the levels in untreated mice. In an OGTT, at the same dose, a 30% fall in PPPG was observed after 3h, in normal, sub-diabetic, and mildly diabetic rats. A 21-day treatment of severely diabetic rats with the M. oleifera extract at a daily dose of 300mg/kg-bw reduced FPG and PPPG by 69 and 51%, respectively, relative to untreated controls. In all the above experiments, the hypoglycemic effect of the plant extract was comparable to that of the anti-diabetic drug Glipizide administered at 2.5mg/kg-bw.

Human studies

In a controlled study with untreated T2DM patients, William et al. (1993) examined howM. oleifera addition to a standardized meal, taken after an overnight fast, affected the 1- and 2-h PPPG, relative to the standard meal alone or a 75-g oral glucose load. M. oleifera was compared to bitter gourd (Momordica charantia) and curry leaves (Murraya koenigii). Compared to the glucose load, standard meals with or without vegetable supplements induced a significantly lower rise in PPPG (glycemic response) as derived from AUCs. However, when leaf-supplemented meals were compared to standard meals, only the M. oleifera leaf-supplemented meal elicited a lower response (−21%, P<0.01). Plasma insulin AUCs did not differ significantly between the two meals, suggesting that the hypoglycemic effect of M. oleifera leaf supplementation was not due to increased insulin secretion.

Kumari (2010) examined the hypoglycemic effect of M. oleifera leaf dietary consumption over a 40-day period in T2DM patients, 30–60years of age, not on anti-hyperglycemic medication. The experimental group included 46 subjects, 32 men, and 14 women; the control group of 9 subjects included 4 men and 5 women. Daily meals were comparable among these groups in terms of relative content of food types (e.g., cereals, green leafy vegetables, fruits, etc.) and nutrients (e.g., proteins, fat, fiber, minerals, etc.) as well as calories. The experimental group received a daily dose of 8g M. oleifera leaf powder. FPG and PPPG at the end of the protocol (final) were compared to baseline levels. Final values did not differ much from baseline in the control group. They were significantly reduced in the experimental group (FPG: −28%, P<0.01; PPPG: −26%, P<0.05).

More recently, Ghiridhari et al. (2011) studied a group of 60 T2DM patients, age 40–58years, BMI 20–25kg/m², on sulfonylurea medication and a standardized calorie-restricted diet. The patients were equally divided into an experimental and a control groups. Patients in the experimental group were prescribed two M. oleifera leaf tablets/day, one after breakfast, the other after dinner for 90days. M. oleifera leaf powder constituted 98% (w/w) of the tablet content, but the average weight of tablets was not specified, making the total daily dose unclear. Blood glycated hemoglobin (HbA_1c) was measured before and after the regimen. PPPG was determined before the regimen and every 30days afterward. In the control group, HbA_1c and PPPG progressed downwardly with time, but the change was not significant. In the experimental group, in contrast, relative to the baseline, HbA_1c decreased by 0.4% point (from 7.8±0.5 to 7.4±0.6; P<0.01). Compared to the starting levels (210±49mg/dL), PPPG in the experimental group progressively decreased with treatment duration, by 9% after 30days, 17% after 60days, and 29% after 90days (P<0.01), indicating that M. oleifera medication can induce with time better glucose tolerance. However, it should be noted that treatment allocation to patients appear to have not been randomized as baseline values for the two parameters were higher in the experimental group than in the control group, 7.8±0.5 vs. 7.4±0.6% for HbA_1c, 210±49 vs. 179±36mg/dL for PPPG.

Go to:

THERAPEUTIC POTENTIAL OF M. OLEIFERA IN DYSLIPIDEMIA

Lipid homeostasis

Lipids constitute a major class of hydrophobic constituents of the body. Their main forms are cholesterol, phospholipids (PL), and triglycerides (TG). Lipids are involved in a variety of biological processes, including membrane formation, intracellular and intercellular signaling, as well as energy storage and production. The body derives its lipids from de novo cellular biosynthesis and from nutrition. Cellular biosynthesis of lipids is regulated at the transcriptional level by sterol-regulated element-binding proteins (SREBPs) 1 and 2. SREBP-1 promotes the biosynthesis fatty acids and TG, SREBP-2 that of cholesterol (Horton, 2002).

Intestinal and plasma lipids are transported by lipoproteins particles. Apolipoproteins (Apo) constitute the protein components of these particles. Lipoproteins vary in density and, depending on their relative contents in TG, cholesterol, and PL, are identified as chylomicrons, very low-density lipoprotein (VLDL), LDL, intermediate density lipoprotein (IDL), and high-density lipoprotein (HDL). Lipids are transported by chylomicrons in the intestinal lymphatic system; and, in the bloodstream, by chylomicron remnants, VLDL, LDL, IDL, and HDL (Abeles et al., 1992; Havel and Kane, 2001).

The liver plays a pivotal role in lipid metabolism. It extracts cholesterol from intestinal chylomicrons and excretes it back into the intestines with bile acids. It biosynthesizes TG and cholesterol and packages them as VLDL, that it secretes into the bloodstream. Through the LDL receptor (LDLR), it clears up plasma LDL as well as IDL from VLDL or HDL catabolism. HDL mediates the reverse transport of cholesterol from extra-hepatic tissues to the liver (Havel and Kane, 2001). Liver LDLR levels and its capacity to clear blood LDL are down-regulated by proprotein convertase subtilisin/kexin-type 9 (PCSK9), a plasma protein secreted by this organ (Horton et al., 2009).

Dyslipidemia

Dyslipidemia is a disorder characterized by alterations in the levels and composition of plasma lipids. According to Adult Treatment Panel III (2001), plasma levels ≥200mg/dL for TC, ≥130mg/dL for LDL-C, <40mg/dL for HDL-C, and ≥150mg/dL for TG are dyslipidemic. Dyslipidemia may result from inborn defects of lipoprotein production or metabolism; but in most cases, it is secondary to an unhealthy lifestyle (e.g., excessive cigarette smoking or alcohol consumption), other health disorders (e.g., obesity, diabetes, infection, obstructive liver disease), or medication (e.g., β blockers, steroids).

Besides hypertension, chronic dyslipidemia is a major cause of atherosclerosis, a vascular disease affecting blood circulation in the coronary, central, and peripheral arteries. The pathology is initiated by irritation of the arterial endothelium by high level of circulating LDL-C, which leads to overexpression of adhesion and chemoattraction molecules (e.g., vascular cell adhesion molecule-1, intercellular adhesion molecule, P and E selectins, monocyte chemoattractant protein-1) to injured sites, and the recruitment and capture of circulating monocytes to these sites. These immune cells penetrate into the sub endothelium and differentiate into tissue macrophages, which take up oxidized LDL (oxLDL) via scavenger receptors (e.g., CD36, scavenger receptor-A), becoming the lipid-laden foam cells characteristic of atheromatous plaques. In response to growth factors, resident vascular smooth muscle cells (VSMC) proliferate and form a fibrous cap overlying the plaques. The oxidative process that leads to oxLDL production also contributes to atherogenesis, as this modified lipoprotein and its by-products (oxysterols and oxPL) act as monocyte chemoattractants and VSMC mitogens. Clinical complications of this process include a narrowing of the arterial lumen, plaque rupture, and formation of circulating thrombi. These complications could lead to coronary artery disease (CAD), myocardial infarction, thrombo-embolic stroke, and peripheral artery disease (Steinberg, 2002; Libby et al., 2011).

Over the years, there has been a vigorous debate over the predictive value of plasma LDL-C level as a marker of CVD risk in humans. An emerging view is that the level of non-HDL lipoproteins, also captured in the TC/HDL-C or HDL-C/non-HDL-C ratio, may constitute a better marker, and its reduction a more cogent measure of the efficacy of anti-dyslipidemia therapies (Sniderman et al., 2010; Manickam et al.,2011).

Evidence of anti-dyslipidemic property of M. oleifera

Five studies were identified in the scientific literature: three were conducted with experimental animals (Ghasi et al., 2000; Chumark et al., 2008; Jain et al., 2010), two with human subjects (Kumari, 2010; Nambiar et al., 2010). They are summarized in Table ​Table22.

Table 2

Moringa oleifera experimental therapy for chronic hyperlipidemia.

Animal studies

Chumark et al. (2008) examined the therapeutic potential of M. oleifera leaves on dyslipidemia induced in rabbits on a high-cholesterol (5%) diet (HCD) for 12weeks. By the end of the regimen, relative to rabbits on a normal diet, HCD-fed rabbits experienced several-fold (×) increases in the plasma levels of total cholesterol (TC, 55×), HDL-C (17×), LDL-C (131×), and TG (4×). The diet also caused extensive plaque formation in carotid arteries. When these HCD rabbits were concomitantly fed aM. oleifera aqueous leaf extract, at the daily dose of 100mg/kg-bw for the duration of the protocol, these increases were reduced: for TC and lipoprotein-cholesterol by about 50%, for TG by 75%, and for carotic plaque formation by 97%. This protective effect was comparable to that of the anti-cholesterol drug simvastatin, given p.o., at a daily dose of 5mg/kg-bw. Similar results were also obtained with HCD rabbits fed an aqueous extract of M. oleifera fruits (Mehta et al., 2003).

The anti-dyslipidemic effects of M. oleifera leaves were also examined in rats fed a high-fat diet (HFD). In one study (Ghasi et al., 2000), Wistar rats were fed, for 30days, a HFD containing 16% (w/w) fat, with or without an aqueous extract of M. oleifera leaves at a daily dose of 1g/kg-bw. In untreated rats, the diet caused a 30% increase in plasma TC. In treated rats, the increase was reduced to 14%. In another study (Jain et al., 2010), albino rats were fed, for 30days, a HFD containing 26% fat, with or without a methanolic extracts of M. oleifera leaves at daily doses of 150, 300, or 600mg/kg-bw. In untreated rats, the diet increased plasma TC (2.4×), LDL-C (7.7×), VLDL-C (1.7×), and TG (1.6×). At the highest dose,M. oleifera treatment reduced these increases to 1.5×, 2.2×, 1.3×, and 1.3×, respectively (P<0.01). Interestingly, serum HDL-C was unchanged by HFD diet alone; it was increased 2.4× in rats fed leaf extract-supplemented HFD, significantly reducing the TC/HDL-C ratio.

Human studies

Nambiar et al. (2010) examined the potential anti-dyslipidemic effect of M. oleifera in 35 hyperlipidemic subjects (TC>180mg/dL or TG>140mg/dL), 26 men and 9 women. The control and experimental groups consisted of 18 subjects and 17 subjects, respectively. Anthropometric values (age, height, weight, body mass index, waist/hip ratio) within gender were similar between the two groups, as was their daily nutrient intake. The experimental group consumed a daily total of 4.6g of dehydrated M. oleifera leaves, as four 550-mg tablets twice daily, for 50days. Plasma lipid profiles were determined before and after the regimen. Compared to the control group, the experimental group experienced a 1.6% fall in plasma TC (P<0.05) and a 6.3% increase of HDL-C, with non-significant trends toward lower LDL-C, VLDL-C, and TG. However, relative to baseline, final non-HDL-C and TC/HDL-C values decreased by 3.7 and 6.6%, respectively (P<0.001), indicating that the treatment induced a lesser atherogenic lipid profile.

In the study of T2DM patients reported by Kumari (2010), the corrective effect of M. oleifera dietary leaves on dyslipidemia was also examined. Compared to the control group, the experimental group receiving 8g of M. oleifera leaf powder daily for 40days experienced a significant fall in the plasma levels of TC (−14%), LDL-C (−29%), VLDL-C (−15%), and TG (−14%; P<0.05 to <0.01). HDL-C increased by 9% (non-significant), but the HDL-C/non-HDL-C ratio increased by 37% (P<0.01).

Go to:

PHARMACOLOGY OF M. OLEIFERA LEAVES

Broad-spectrum physiological properties

Because of the chemical complexity of the M. oleifera medicinal formulations used in the studies reviewed above, their apparent therapeutic effects could be due to the combined actions of various bioactive components found in the plant, including trace metal ions, vitamins, alkaloids, carotenoids, polyphenols, fats, carbohydrates, and proteins (Coppin, 2008; Amaglo et al., 2010). Some compounds may collectively affect broad aspects of physiology, such as nutriment absorption and processing, redox state, or immunity.

Anti-nutrient properties

Moringa oleifera leaves contain phytosterols such as β-sitosterol (Jain et al., 2010). These compounds can reduce intestinal uptake of dietary cholesterol (Lin et al., 2010). They could partly account for the decrease of plasma cholesterol and the increase of fecal cholesterol observed in rodents treated with M. oleifera leaves (Mehta et al., 2003; Jain et al., 2010). M. oleifera leaf powder also contain about 12% (w/w) fibers (Joshi and Mehta, 2010). Dietary fibers reduce gastric emptying (Bortolotti et al.,2008). They may partly explain the greater stomach content, the improved OGTT response in treated GK diabetic rats (Ndong et al., 2007b), as well as the progressive improvement of PPPG levels in treated T2DM patients (Ghiridhari et al., 2011).

Antioxidant properties

The viability and functionality of a cell partly depends on a favorable redox state, i.e., on its ability to prevent excessive oxidation of its macromolecules, including DNA, proteins, and lipids (Ryter et al., 2007; Limon-Pacheco and Gonsebatt, 2009). ROS and free radicals are the major mediators of the oxidative process. Cellular inability to reduce ROS leads to oxidative stress. All cells are variably capable of endogenous self-protection against this stress through the actions of enzymes such as catalase, superoxide dismutase, and glutathione peroxidase, as well as through reducing molecules such as glutathione. Nutritional antioxidants such as vitamins A, C, and E provide additional protection from the stress (Limon-Pacheco and Gonsebatt, 2009).

Oxidative stress is widely accepted as a major contributing factor in the pathogenesis of CVD and diabetes (Dhalla et al., 2000; Kaneto et al., 2007; Rodrigo et al., 2011). A recurring explanation for the therapeutic actions of M. oleifera medication is the relatively high antioxidant activity of its leaves, flowers, and seeds (Chumark et al., 2008; Sreelatha and Padma, 2009; Verma et al., 2009; Atawodi et al., 2010). Among the major classes of phytochemicals found in the plant, flavonoids appear to carry most of this activity.

Anti-inflammatory properties

Inflammation with its wide array of cytokines secreted by immune cells is an integral part of the pathophysiology of obesity, hypertension, atherosclerosis, and diabetes (Rana et al.,2007). Extracts from M. oleifera leaves have been shown to modulate humoral and cellular immunity in rats and mice (Gupta et al., 2010; Sudha et al., 2010). They have exhibited strong anti-inflammatory properties in rodent models of chemically induced inflammation of the paw (Sulaiman et al., 2008; Mahajan and Mehta, 2009). These properties have been more extensively studied with fruit and seed extracts (Cheenpracha et al., 2010; Mahajan and Mehta, 2010; Muangnoi et al., 2011). They may also contribute to the observed anti-atherogenic and anti-diabetic effects of M. oleifera therapy.

Bioactive phytochemicals

An informative historical account of research in the phytochemistry of M. oleifera prior to 1995 can be found in Saleem’s doctoral thesis available on line (Saleem, 1995). Since then, the research has been expanded and refined, not only on the chemical structures of plant molecules, but also on their nutritional and medicinal properties. Of major medicinal interest are three structural classes of phytochemicals: glucosinolates, flavonoids, and phenolic acids (Saleem, 1995; Bennett et al., 2003; Lako et al., 2007; Manguro and Lemmen, 2007; Coppin, 2008; Amaglo et al., 2010; Kasolo et al., 2010). Their content in M. oleifera leaves varies somewhat with the geographic and climatic conditions under which the plant was grown, as well as with the processing methods for the collected leaves (Bennett et al., 2003; Coppin, 2008; Mukunzi et al., 2011).

Glucosinolates are characterized by β-thioglucoside N-hydroxysulfate motif (Figure ​(Figure2A).2A). In M. oleiferaleaves, most phytochemicals of this class carry a benzyl-glycoside group linked to the single carbon of the motif. The most abundant of them is 4-O-(α-l-rhamnopyranosyl-oxy)-benzylglucosinolate, otherwise known as glucomoringin (Amaglo et al., 2010). Enzymatic hydrolysis of the glucosinolate motif of members of this class leads to the formation of corresponding isothiocyanates, thiocyanates, or nitriles. Several of these by-products have been shown to possess antihypertensive properties (Faizi et al., 1992,1994, 1998).

Figure 2

Structural motifs and backbones of major phytochemicals found M. oleifera leaves. (A) Glucosinolates and their metabolites. (B) Flavonol major derivatives. (C) Phenolic acids. Their known derivatives or metabolites are indicated.

Flavonoids and phenolic acids are collectively referred to phenolic compounds. The structural skeleton of flavonoids is made of two aromatic rings joined by a three-carbon link; that of the sub-class of flavonols is 3-hydroxy-2-phenylchromen-4-one (Figure ​(Figure2B).2B). Quercetin and kaempferol, in their as 3′-O-glycoside forms, are the predominant flavonols in M. oleifera leaves. The sugar moieties include, among others, rhamnoglycosyl (rutinosides), glucosyl (glucosides), 6′ malonyglucosyl, and 2′-galloylrutinoside groups (Bennett et al., 2003; Manguro and Lemmen, 2007; Amaglo et al., 2010). Biologically, flavonoids are best known for their antioxidant properties, but their metabolic pathways of activity remain to be fully elucidated (Rice-Evans, 2001). Phenolic acids have benzoic acid and cinnamic acid as backbones, with one or several hydroxyl groups (Figure ​(Figure2C).2C). Chlorogenic acid, which is an ester of dihydrocinnamic acid (caffeic acid) and quinic acid, is a major phenolic acid in M. oleifera leaves (Bennett et al., 2003; Amaglo et al., 2010).

Four of the best-characterized phytochemicals for their therapeutic efficacy in hyperglycemia, dyslipidemia, or related physiological conditions are shown in Figure ​Figure33.

Figure 3

Some bioactive phytochemicals found M. oleifera leaves. Their structures were obtained from the online in the PubChem database at www.pubmed.ncbi.nlm.nih.org/.

Quercetin

The flavonol quercetin is found at concentrations as high as 100mg/100g of dried M. oleiferaleaves (Lako et al., 2007), predominantly as quercetin-3-O-β-d-glucoside also known as isoquercitrin or isotrifolin (Bennett et al., 2003; Atawodi et al., 2010; Figure ​Figure3A).3A). Quercetin is a potent antioxidant (Zhang et al., 2011) with multiple therapeutic properties (Bischoff, 2008). It can reduce hyperlipidemia and atherosclerosis in HCD or HFD rabbits (Juzwiak et al., 2005; Kamada et al., 2005). It has shown anti-dyslipidemic, hypotensive, and anti-diabetic effects in the obese Zucker rat model of metabolic syndrome (Rivera et al., 2008). It can protect insulin-producing pancreatic β cells from STZ-induced oxidative stress and apoptosis in rats (Coskun et al., 2005). Its hypotensive effect has been confirmed in a randomized, double-blind placebo-controlled, human study (Edwards et al., 2007).

Chlorogenic acid

Chlorogenic acid (Figure ​(Figure3B)3B) can beneficially affect glucose metabolism. It has been shown to inhibit glucose-6-phosphate translocase in rat liver, reducing hepatic gluconeogenesis and glycogenolysis (Hemmerle et al., 1997; Karthikesan et al., 2010a). It was found to lower PPBG in obese Zucker rats (Rodriguez de Sotillo and Hadley, 2002). In OGTT experiments performed on rats or humans, it reduced the glycemic response in both species (van Dijk et al., 2009; Tunnicliffe et al., 2011); in rodents, it also reduced the glucose AUC (Tunnicliffe et al., 2011). Its anti-dyslipidemic properties are more evident as its dietary supplementation has been shown to significantly reduce plasma TC and TG in obese Zucker rats or HFD mice (Rodriguez de Sotillo and Hadley, 2002; Cho et al., 2010) and to reverse STZ-induced dyslipidemia in diabetic rats (Karthikesan et al., 2010b).

Moringinine

The alkaloid moringinine was initially purified from M. oleifera root bark (Ghosh et al., 1935) and later chemically identified as benzylamine (Chakravarti, 1955; Figure ​Figure3C).3C). It is also present in leaves. This substance was suspected to mediate the hypoglycemic effect of the plant. An early study showed that Wistar rats provided with drinking water containing 2.9g/L of benzylamine for 7weeks exhibited a reduced hyperglycemic response in an intraperitoneal glucose tolerance test (IPGTT), suggesting improved glucose tolerance (Bour et al., 2005). More recently, the effect was further explored using HFD-fed, insulin-resistant C57BL/6 mice taking an estimated daily dose 386mg/kg-bw in drinking water for 17weeks. Compared to untreated controls, these mice gained less weight, had reduced FPG and plasma TC, and were more glucose tolerant (Iffiu-Soltesz et al., 2010).

Niaziminin

Niaziminin (Figure ​(Figure3D)3D) is a mustard oil glycoside initially isolated (along with other glycosides such as niazinin and niazimicin) from ethanolic extracts of M. oleifera leaves, based on their hypotensive properties on Wistar rats. At 1mg and 3mg/kg-bw, these compounds caused a 16–22 and a 40–65% fall of mean arterial blood pressure (MABP), respectively (Faizi et al., 1992). Other active isothiocyanate glycosides and thiocarbamates were isolated from the plant using the same bioassay (Faizi et al., 1994,1998; Saleem, 1995).

Aurantiamide acetate

This compound was isolated from M. oleifera roots and structurally identified as N-benzoylphenylalanyl phenylalinol acetate (Figure ​(Figure3E).3E). At 25μM, this unusual dipeptide derivative inhibited by nearly 90% the secretion TNFα and IL-2 from lipopolysaccharide-stimulated peripheral blood lymphocytes in culture. It had no effect on IL-6 secretion (Sashidhara et al., 2009). This inhibitory activity may contribute to the anti-inflammatory properties of the plant.

Go to:

TOXICOLOGY OF M. OLEIFERA THERAPY LEAVES

In the studies reviewed in this report, no acute or sub-acute toxicity was reported following treatment with M. oleifera leaves at the dosage used. The toxicology of this phytotherapy was specifically addressed in two studies. Using rats, Adedapo et al. (2009) examined the safety of an aqueous extract of M. oleiferataken orally at 400, 800, 1600, and 2000mg/kg-bw (n=6 rats/dose). The treatment was either acute (single dose followed by a 48-h observation period), or sub-acute (daily doses, except the highest, for 21days followed by hematological profiling). Acute treatment caused no death at 400 or 800mg/kg-bw; it caused 1/6 and 2/6 deaths at 1600 and 2000mg/kg-bw, respectively. At these high doses, surviving rats exhibited transient dullness for up to 5h. Sub-acute treatment with 400mg/kg-bw caused a modest (10%), but significant (P<0.05) increase in packed cell volume (PCV) and white-blood cell (WBC) count; with 800mg/kg-bw, it lowered the PCV, but increased the WBC; with 1600mg/kg-bw, it lowered both parameters. The WBC changes affected lymphocytes, neutrophils, and monocytes, but not eosinophils. Blood levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were also examined as surrogate indicators of hepatic function. The results were inconsistent: treatment with 400 and 1600mg/kg-bw increased AST and ALT levels by 40–50%; with 800mg/kg-bw, it lowered it by about 50%. A 70% increase in ALP levels was observed only after treatment with 1600mg/kg-bw. Histological examination of tissue sections stained with hematoxylin and eosin revealed no alteration in testis or kidney; a diffuse tissue degeneration was noted in liver. Interestingly, a dose-dependent reduction of body weight gain was noted at the term of the sub-acute treatment with M. oleifera extract: whereas untreated rats gained 53% more weight, those treated with 400, 800, 1600mg/kg-bw of extract gained only 37, 27, and 18%, more, respectively. It is unclear whether this effect is symptomatic of an underlying pathology or indicative of a potential anti-obesity property of the plant. Considering the alterations induced by the sub-acute treatment to be mild, the investigators concluded that nutritional and therapeutic consumption of M. oleifera leaves at doses below 2g/kg-bw is safe.

Other investigators have examined the effects of hexane extracts of M. oleifera leaves on the reproductive organs of male mice after an oral gavage at 17, 170, and 1700mg/kg-bw/day for 21days. They observed a dose-dependent significant increase in testis and epididymis weights, in seminiferous tubule diameter and in epididymal epithelium thickness, but no change in the plasma levels of gonadotropins. They concluded that these changes reflected enhanced spermatogenesis (Cajuday and Pocsidio, 2010).

Several studies using experimental rodent models have shown that M. oleifera leaf extracts can protect the liver from chemically induced damage (Pari and Kumar, 2002; Ndong et al., 2007a; Fakurazi et al.,2008; Buraimoh, 2011).

Go to:

CONCLUSION AND PERSPECTIVES

Collectively, the studies described in this review provide compelling, albeit very preliminary, experimental evidence of a therapeutic potential of M. oleifera leaves in chronic hyperglycemia and dyslipidemia. No report contradicting this evidence was found in the scientific literature. A common weakness of all the human studies reviewed herein was the fact that none was randomized and double-blind, raising the possibilities of bias in the allocation of patients to control and experimental groups.

In spite of converging results, a comparative analysis of these studies is made difficult by variations in agro-climatic conditions of plant growth and harvesting, in leaf processing methods, in final galenic formulations, and in therapeutic protocols. The phytochemical composition of M. oleifera parts have been shown to vary significantly among regions and seasons (Iqbal and Bhanger, 2006; Juliani et al., 2009). Leaf extraction solvents determine the nature and the relative concentrations of bioactive or medicinal ingredients found in final galenic formulations (Siddhuraju and Becker, 2003).

Evaluation of the phytotherapy was often restricted to a narrow set of parameters of disease. For examples, most studies of the anti-diabetic effects were limited to measures of FPG and PPPG levels. Fasting and post-OGTT insulin levels were almost never examined to verify whether the treatment improved the ability of the endocrine pancreas to produce and secrete this hormone in response to elevated blood glucose. Insulin clamp studies would have clarified whether the treatment increased tissue sensitivity to the hormone. Since the medication was administered p.o., measures of plasma levels of incretins and other enterohormones would have given insights on how GIT physiology is affected by the treatment.

In the same vein, it is unclear whether the hypocholesterolemic effect of the therapy was associated with repressed de novo cholesterol biosynthesis (a statin-like effect), increased hepatic cholesterol clearance (statin-like or anti-PCSK9-like effects) or increased intestinal excretion. It might be interesting to find the physiological basis of the cardioprotective increase of the TC/non-HDL-C ratio following treatment. A more extensive analysis on circulating biochemical markers of inflammation would be justified, as the balance of pro and anti-inflammatory cytokines could influence the course of both diabetes and atherosclerosis.

Besides a rationalized investigation of the therapeutic effect of M. oleifera leaves, it is possible to conduct an unbiased search for the affected physiological pathways. This could be achieved by globally comparing tissues or fluids of untreated and treated animals or humans, using the powerful techniques of systems biology, the so-called “omics” (transcriptomics, proteomics, and metabolomics), combined with the equally powerful algorithms of modern bioinformatics (Zhang et al., 2010).

Further exploration of the potentials and the eventual use of M. oleifera therapy in humans will require that reference standards be set for its cultivation, the collection of its parts, as well as for their final conditioning under good agricultural and collection practices (GACP). Such guidelines, specifically directed toward medicinal plants have been defined by the WHO (2003). Furthermore, when the pharmacological basis of the therapeutic effects on a particular pathology is rationally well circumscribed, standardized assays should be required to evaluate all galenic lots for the relevant properties. For example, on the basis of current knowledge, it would indicated that the content in fibers, phytosterols as well in antioxidant and anti-inflammatory activities be evaluated and documented in M. oleifera galenic formulations destined to the treatment of atherosclerosis or diabetes. The shelf life of these properties under specified storage conditions should also be established.

In conclusion, based on the available experimental evidence, M. oleifera leaf powder holds some therapeutic potential for chronic hyperglycemia and hyperlipidemia. However, before it is advocated in any formulation for the treatment of these metabolic disorders in humans, state-of-the art clinical studies must be conducted to establish the consistency of its medicinal efficacy and the safest modalities of its administration.

Go to:

CONFLICT OF INTEREST STATEMENT

The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Go to:

ACKNOWLEDGMENTS

The author thanks Drs. Ajoy Basak, Michel Chrétien, Hugues Loemba, and Teik Chye Ooi for their critical review of the manuscript. He is also grateful to Ms. Denise Joanisse for her invaluable assistance in bibliographical search. The work was supported by grants from the Canadian Institute of Health Research and from the Strauss Foundation.

Go to:

ABBREVIATIONS

ACP, acid phosphatase; ALP, alkaline phosphatase; ALT, alanine aminotransferase; AST, aspartate amino transferase; AUC, area under the curve; bw, body weight; CAD, coronary artery disease; CVD, cardiovascular disease; DM, diabetes mellitus; FPG, fasting blood glucose; GIT, gastrointestinal tract; GK, Goto-Kakizaki; HbA_1c, glycated hemoglobin; HCD, high-cholesterol diet; HDL, high-density lipoprotein; HFD, high-fat diet; IL, interleukin; IPGTT, intraperitoneal glucose tolerance test; LDL, low-density lipoprotein; LDLR, LDL receptor; NEFA, non-esterified fatty acid, OGTT, oral glucose tolerance test; oxLDL, oxidized LDL; PCSK9, proprotein convertase, subtilisin/kexin-type 9; PPPG, post-prandial blood glucose; PVC, packed cell volume; ROS, reactive oxygen species; SREBP, sterol regulatory element-binding protein; STZ, streptozotocin; T2DM, type-2 diabetes mellitus; TG, triglyceride; TNF, tumor necrosis factor; VLDL, very low-density lipoprotein; VSMC, vascular smooth muscle cell; WBC, white-blood cell.

Go to:

sources from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290775/